外源亚精胺对干旱胁迫下甜瓜幼苗抗旱性影响的生理生化机制

Physiological and Biochemical Mechanism of Exogenous Spermidine Improving Drought Resistance in Melon Seedlings Under Drought Stress

摘要: 以“甜脆王”甜瓜幼苗为试验材料，采用营养液培养法，研究20%PEG 6000渗透胁迫下1 mmol·L^-1亚精胺（Spd）对甜瓜幼苗抗旱的调控机制，以期为多胺在抗旱方面的研究提供参考依据。结果表明：1 mmol·L^-1 Spd能够提高20%PEG 6000胁迫下超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性，降低丙二醛含量，提高叶绿素、脯氨酸、可溶性糖和可溶性蛋白质含量。表明外源Spd起到缓解干旱胁迫对甜瓜幼苗伤害的作用，增强甜瓜幼苗对干旱逆境的适应性。

Abstract: ‘Tiancuiwang’ melon seedlings was used as experimental matireials by nutrient solution culture experiments.The regulation mechanism of 1 mmol· L^-1 spermidine（Spd） on drought resistance of melon seedlings under 20% PEG 6000 osmotic stress was studied,in order to provide reference for the study of polyamines in drought resistance.The results showed that spraying 1mmol·L^-1 Spd under drought stress,activities of SOD,POD and CAT could be significantly increased.The contents of chlorophyll,proline,soluble sugar and soluble protein were improved.The content of MDA was reduced content.It indicated that exogenous Spd treatment could alleviate the damage caused by drought stress and enhance the tolerance of melon seedlings to drought environments.