Abstract: The cucumber seeds of ‘Hanyan 28-6’ were used as test materials, water was used as control（CK）,cucumber seeds were soaked and irrigated with 7.2×10~7,7.2×10~6,7.2×10~5,3.6×10~5 CFU·mL^-1four concentrations of B.subitilis BSCY-1 bacterial suspension, to explore the effects of different concentrations of BSCY-1 bacterial suspension on seed germination, as well as morphological and physiological characteristics of seedlings.The growth-promoting effect of endophytic B.subtilis BSCY-1 isolated from strawberry leaves on cucumber was preliminarily clarified, in order to provide reference for the addition of B.subtilis in fertilizer.The results showed that compared with CK,after 7 days and 14 days of root irrigation, 3.6×10~5 CFU·mL^-1 BSCY-1 treatment significantly increased the leaf area and fresh weight of cucumber seedlings.After 7 days and 14 days of root irrigation with BSCY-1,the activities of CAT and POD of 7.2×10~5 CFU·mL^-1 and 3.6×10~5 CFU·mL^-1 treatments were significantly higher than those in CK.SOD activity of 7.2×10~5,3.6×10~5 CFU·mL^-1 BSCY-1 treatments were significantly higher than that of CK after 14 days and 21 days.After 14 days of irrigation with BSCY-1,the soluble starch content, soluble sugar content and chlorophyll content of 3.6×10~5 CFU·mL^-1 treatment were significantly higher than those of the control.In summary, B.subtilis BSCY-1 could improve the physiological and biochemical characteristics of cucumber seedlings, thereby promoting seedling growth, especially the growth-promoting effect of 3.6×10~5 CFU·mL^-1 BSCY-1 treatment was the most obvious.