Abstract: Taking the fermented traditional Chinese medicine dregs as test material,the dilution coating plate method was used for primary screening,and then the plate confrontation method was used for re-screening.The morphological characteristics,physiological and biochemical characteristics,and 18 S rDNA molecular biological characteristics of fungi with antagonistic properties were studied.The inhibitory ability of the antagonistic fungi against Rhizoctonia solani was also tested,in order to utilize the resources of the biocontrol bacteria in the residues of traditional Chinese medicines.The results showed that seven antagonistic fungi with inhibitory effect on Rhizoctonia solani were obtained from the dregs of traditional Chinese medicine,F-1 was determined to be Penicillium citrinum,F-2 was Aspergillus flavus,F-3 was Talaromyces columbinus,F-4 was Aspergillus spinulosporus,F-5 was Amesia atrobrunnea,F-6 was Aspergillus fumigatus,F-7 was Albifimbria viridis.