Abstract: In this study,the effects of exogenous Spm on the iduction of regenerative organs and vitrification of callus with different properties of Lycium ruthenicum Murr.by add different concentrations of Spm into MS medium,in order to provide reference for plant regeneration technology and genetic transformation of Lycium ruthenicum Murr.The results showed that the adding of Spm in appropriate concentrations could increase the rate of callus regeneration and reduce the occurance of vitrification significantly.Compared the effects of Spm on four different types of callus,BEC had the best result,followed by LEC,SEC and AEC.For BEC of QH-N and NX-Z,the optimal concentration of Spm was 30 mg·L-1,the regeneration rate was 60.62% and 63.89%,respectively,and there was no vitrified seedling.The optimal concentration of LEC adding Spm was 20 mg·L-1,the regeneration rate reached 49.48%and 52.48%,respectively,and there was no vitrified seedling.When adding Spm in concentration of 10 mg·L-1 and 15 mg·L-1,SCE had regeneration rate of 33.60%and 42.11%,and the vitrification rate was reduced to 1.14%and 0,respectively.For AEC,the best concentration of adding Spm was 15 mg·L-1.The regeneration rate could reach to 19.89% and 34.86%,and the vitrification rate could reduced to 12.19% and 8.97%,respectively.These results indicated that exogenous spermine could promote the regeneration rate of callus,and reduce the occurrence of vitrified seedlings in different degrees.