Abstract: Taking the rhizosphere soil and root tissue of Paris polyphylla in different habitats as test materials, high-throughput sequencing and bioinformatics methods were used to study the composition of the AMF community of P.polyphylla in different habitats, and correlation analysis was conducted on soil environmental factors, in order to provide reference for the development of specialized bacterial agents for P.polyphylla and the improvement of cultivation efficiency.The results showed that both NMDS analysis and sample level clustering showed that only WNT was relatively independent, while other samples were interleaved.The diversity of root AMF was ZJG＞WNG（DFG）,rhizosphere soil was ZJT＞WNT＞DFT,and the total OTU was 0.The top 11 species with abundance showed a significant positive correlation with ACP,OM,SC,and a significant negative correlation with TP,AP,AK,PPO,TK,pH,URE;The bacteria with positive correlation were all distributed in ZJ samples, and the relationship was more closely related.Through NCBI sequence alignment, strains such as Septoglomus constrictum,Claroideoglomus etunicatum,Acaulospora brasiliensis,Funneliformis mosseae,Scutellospora calospora,and Acaulospora koskei were obtained.The aggregation of AMF was influenced by the interweaving of geographical distance and local environmental factors.The microbial community structure was influenced by the combined effects of altitude, habitat, and soil physicochemical factors.Fungi such as Acaulospora brasiliensis and Funneliformis mosseae were of great significance for the growth and development of P.polyphylla.These strains could be used to develop biological agents to improve the cultivation efficiency of P.polyphylla.