Abstract: Taking 11.7 cm Phalaenopsis ‘Big Chili’ as experimental material, seven different substrates were used to grow Phalaenopsis,and growth parameters were measured, the effects of different substrates on the growth and development of Phalaenopsis ‘Big Chili’ were studied, in order to provide reference for the selection and utilization of substrates.The results showed that the growth condition of Phalaenopsis cultivated with imported water moss, domestic water moss and danish peat were the best, the growth condition of Phalaenopsis cultivated with dutch peat and domestic water moss∶corncob mushroom residue=7∶3 were better, and the growth condition of Phalaenopsis cultivated with sawdust mushroom residue and corncob mushroom residue were the worst.In the production and cultivation, Danish peat could become a substitute to replace domestic water moss and imported water moss as the substrate of ‘Big Chili’ 11.7 cm seedlings.