Abstract: The suspected Phallus dongsun strain collected from Longli county, Guizhou Province was used as the test material, and its molecular identification was carried out by ITS sequence analysis, and the influences of different substances on the growth of mycelium were studied by single factor experiments, in order to clarify its taxonomic status, and provide reference for artificial cultivation of Phallus dongsun and follow-up in-depth research.The results showed that the length of the ITS sequence was 576 bp, which had the highest similarity with Phallus dongsun,and the strain was identified as Phallus dongsun.The best carbon source for the growth of the liquid strain of this strain was fructose, followed by glucose; the best nitrogen source was yeast extract, followed by beef extract; the best main material for the growth of cultivar was peach tree, followed by birch tree; and the best auxiliary material was bagasse, followed by corn cob.