Abstract: Taking Iris sanguinea as materials,the method of waterlogging treatment was adopted.Effects of different waterlogging time on the growth and physiology characters of Iris sanguinea were studied,in order to illuminate the response to waterlogging stress of Iris sanguinea,and provide a reference for the application of Iris sanguinea in garden and germplasm resources innovation.The results showed that the plant height of Iris sanguinea was lower than the control,and root length was siginificantly less than that of control when they were waterlogged for 20 days.As the waterlogging time increased,the content of MDA reached the maximum,and then declined when waterlogged for 8 days.The activity of SOD increased,the activity of POD first increased and then decreased.The proline content first increased and then decreased.The soluble sugar reached the minimum when waterlogged for 8 days then increased slowly.The soluble protein content increased in the early stage,then decreased,and increased again.In conclusion,the growth of Iris sanguinea is inhibited by waterlogging stress.But it has a certain tolerance to waterlogging stress and can adapt to wetland environments.