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四种中药提取物对‘红阳’猕猴桃软腐病菌拟茎点霉菌的抑制作用

Inhibitory Effects of Four Traditional Chinese Medicine Extracts on Postharvest Fungal Phomopsis sp.in ‘Hongyang’ Kiwifruit

  • 摘要: 以‘红阳’猕猴桃为试材,采用菌丝生长速率法,研究了不同浓度八角茴香、花椒、刺梨和两头毛乙醇提取物对拟茎点霉菌的抑制效应,以期探究中药提取物对‘红阳’猕猴桃软腐病的防治作用。结果表明:这4种中药提取物对拟茎点霉菌均表现出了明显的抑制作用,但是在相同浓度下它们的抑制效果有所差异。如在低浓度12.5 mg·mL-1时,八角茴香、花椒、刺梨和两头毛的抑菌率分别为46.82%、53.57%、38.48%和50.80%。而在高浓度100 mg·mL-1时,其抑菌率分别为86.11%、83.73%、66.67%和82.14%。另外,这4种中药提取物对供试菌种菌丝的EC50值分别为15.64、8.76、28.48 mg·mL-1和9.77 mg·mL-1。八角茴香提取物的室内防治效果明显。综上,这4种中药提取物对拟茎点霉菌的抑制作用随着浓度的增加而呈现不同程度的增加,将来可以作为植物杀菌剂资源。

     

    Abstract: The inhibitory effects of the ethanol extracts of Illicium verum,Zanthoxylum bungeanum,Rosa roxburghii and Incarvillea argutaat the different concentrations (0,12.5,25.0,50.0mg·m L-1and 100.0 mg·mL-1)against the pathogen,one of the postharvest fungi in ‘Hongyang’ kiwifruit,were determined by mycelial growth rate method.The results showed that the ethanol extracts of these Chinese medicine could apparently inhibit the mycelial growth of Phomopsis sp.,but they showed the different inhibitory effects at the same concentration.When the lowest concentration of extracts was 12.5 mg·mL-1,the inhibitory rates of I.verum,Z.bungeanum,R.roxburghii and I.arguta were 46.82%,53.57%,38.49% and 50.80%,respectively.When the concentration was 100.0mg·mL-1,their inhibitory rates were 86.11%,83.73%,66.67% and 82.14% with EC50 values of15.64,8.76,28.48mg·mL-1and 9.77 mg·mL-1,respectively.These results in dicated that all four traditional Chinese medicine extracts showed different increasing of inhibitory effects on Phomopsissp.with increasing of concentration and can be used as future plant fungicides.

     

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