Abstract: The full length of GPPS was cloned from Paeonia lactiflora with its root as material by RT-PCR technique and its sequence was analyzed by bioinformatics,in order to lay a foundation for further study on the function of PlGPPSin the biosynthetic pathway of terpenoids.The results showed that the full length cDNA of GPPS was 1 557bp （GenBank accession KP645373）,which encoded 421 amino acids.The molecular weight of GPPS protein was 46.31 kDa,and its pI was 7.63.Bioinformatic analysis indicated that GPPS,located in plasma,was a stable hydrophobic protein without transmembrane domain or signal peptide.BlastP analysis demonstrated that that GPPS had highly similarity with GPPS fromAzadirachta indica.Multiple protein sequence analysis showed that the N-terminus was highly variable.The phylogenetic tree analysis revealed that the genetic relationship of GPPS with those from other related plants was relatively distant.