Abstract: The application of the Internet of Things（IoT） system has become the development direction of modern greenhouses.The IoT protocols were numerous and complex, and each was distributed in various layers.Wireless protocols were mainly used between the IoT sensing devices and the controllers.One type of protocol was suitable for wireless communication at close range, low speed, low power consumption, low cost, and low complexity.The other type of protocol was suitable for wireless communication at long distance, low bit rate, and low power consumption.In the application example of the greenhouse intelligent IoT system based on multi-protocol, according to the demand for kiwi fruit growth environment, comprehensively considering many factors of the greenhouse, the IoT system architecture was designed, and ZigBee, IEEE 802.11 x, TCP/IP and 4 G/5 G was selected reasonably, and, in order to cooperate with the application of multi-protocol, the appropriate IoT equipment were selected.In the example, the sensor network was connected to the controller by the wireless signal transceiver module, and then was connected to the local campus network through the gateway, and then the data was transmitted to the cloud server for storage.On the basis of network communication such as the Internet and mobile Internet, the sensing, transmission and processing functions were realized.The practical application showed that the multi protocol of the Internet of Things work well with stable performance, and the expected effect is achieved.