Abstract: Taking 26 Oudemansiella raphanipes strains collected from the whole country as test materials, the breeding of Oudemansiella raphanipes strains with stress resistance was studied by means of artificial stress resistance domestication and protoplast sports breeding, in order to provide reference for the breeding of Oudemansiella raphanipes excellent strains.The results showed that 26 strains of Oudemansiella raphanipes were collected, after（26 ℃,16 hours; 30 ℃,8 hours）,（26 ℃,16 hours; 32 ℃,8 hours）,（26 ℃,16 hours; 34 ℃,8 hours）,（26 ℃,16 hours; 36 ℃,8 hours） under the condition of six high temperature resistant strains, Sgw, H08 gw, Lgw, Qgw, H09 gw and H01 gw, were screened out.Through the confrontation test between Oudemansiella raphanipes and Trichoderma harzianum strains, three strains of F2,Sgw and Lgw with strong resistance to Trichoderma harzianum were screened.Protoplast fusion was carried out with high temperature resistant domesticated strains（Sgw, H08gw） and Trichoderma harzianum resistant strains（F2,Sgw, Lgw） as female parents, and protoplast fusion was carried out at（26 ℃,16 hours; 32 ℃,8 hours）,and 4 strains with strong stress resistance were screened out, Y1,Y4,Z1 and Z9.Y4,Y1 and Sgw had high temperature resistance, high yield and resistance to Trichoderma harzianumpollution.Through the determination of the enzyme activity index of the stress-resistant strain and the transcriptome analysis of the resistant strain, it was found that the metabolic ability, catalytic activity, cell structure and other functions of the mycelium cells of the Trichoderma harzianum-resistant strain changed, and through signal transduction, carbohydrate metabolism, amino acid metabolism, peroxisome and other metabolic pathways, in order to achieve the effect of resisting Trichoderma harzianum infection and high temperature stress.Y4,Y1 and Sgw strains were the final excellent strains screened in this study, which could be further optimized and cultivated in production.