Abstract: The stem tips of Elsholtzia bodinieri were used as explants and different concentrations of plant growth regulators were used in tissue culture experiments.The effects of different sterilization treatments, media types, hormone concentrations and transplanting substrates on the initial culture, subculture proliferation, rooting culture and seedling transplanting of Elsholtzia bodinieri were studied.In order to lay a foundation for in vitro propagation, in vitro mutagenesis and genetic transformation of Elsholtzia bodinieri.The results showed that the optimum sterilization method for the stem tips of Elsholtzia bodinieri was 75% alcohol for 30 seconds+0.1% HgCl₂ for 6 minutes, and the survival rate was 76.67%.The optimum primary culture medium was MS+6-BA 1.0 mg·L^-1+IAA 0.10 mg·L^-1,and the induction rate was up to 88.89%.The optimum proliferation medium was MS+6-BA 0.3 mg·L^-1+NAA 0.05 mg·L^-1,the proliferation coefficient was 12.91.The best rooting medium was 1/2MS+IBA 1.00 mg·L^-1,with a rooting rate of 97.78%.After 4 days of open cover, the seedlings were transplanted into a mixed substrate of 1 humus soil∶1 perlite∶1 peat, with a survival rate of 91.11%.