Abstract: Eleven strains of Agaricus blazei with excellent performance inside and outside the province were introduced.According to the basic biological research methods, the effects of different medium, light, temperature and pH on the growth of mycelium were studied.Meanwhile, the research on the preservation technology of Agaricus blazei Murrill strain resources was carried out, in order to screen out the most suitable protective agent formula for Agaricus blazei Murrill preservation.The results showed that Agaricus blazei Murrill was suitable for growth on the medium containing wheat bran, the hyphae were suitable for dark conditions, the suitable temperature range was 25-32 ℃,and the suitable acid-base environment was between pH 6-7.Agaricus blazei Murrill mycelium was cylindrical, and the tip of the mycelium was arranged into radial and curly shapes, with less clamp connection and less dense distribution.The optimal formula of aqueous solution protective agent for Agaricus blazei preservation was 25% glycerol and 5% sucrose solution in a ratio of 1∶1.