Abstract: The genetic diversity of 34 germplasm resources of Polygonatum Mill.（Polygonatum cyrtonema,Polygonatum hunanense,Polygonatum praecox,Polygonatum zanlanscianense,Polygonatum kingianum and Polygonatum odoratum） were analyzed by SRAP molecular marker,in order to provide reference for germplamsm identification and molecular breeding of Polygonatum Mill..The results showed that a total of 106 loci were detected using 12 pairs of SRAP polymorphic primers,with a polymorphism percentage of 100.0%.The average Nei’s gene diversity index（H）,the average Shannon diversity index（Ⅰ）,the genetic differentiation coefficient（Gst）,and the gene flow between germplasm was 0.285 8,0.439 9,0.536 0,and 0.432 8,respectively.The genetic similarity coefficient between 34 germplasm ranges from 0.500 0 to 0.952 8,with an average value of 0.669 8.The genetic differences between P.hunanense and P.kingianum germplasm were the smallest,while the genetic differences between P.cyrtonema and P.kingianum germplasm were the largest.Cluster analysis divided 34 germplasm of Polygonatum Mill.into 6 major categories,with P.cyrtonema,P.hunanense,P.praecox,P.zanlanscianense,P.kingianum, and P.odoratum clustered into one category,respectively.The 12 pairs of polymorphic SRAP molecular markers in this study could effectively identify different germplasm of Polygonatum Mill,and provide reference for genetic diversity analysis,germplasm identification,and molecular marker assisted breeding of Polygonatum species.