Abstract: Shoots of Vaccinium bracteatum were used as explants to research the key points in tissue culture,which referring to how to obtain sterile explants,how to generate adventitious buds,and how to rooting,in order to established tissue culture of Vaccinium bracteatum.The results showed that the most effective way to disinfect explants in this experiment was soaking them into 0.1% mercury chloride solution for 10 minutes.It was suitable for inducing adventitious buds of Vaccinium bracteatum in WPM basic medium while it was not suitable for inducing adventitious buds of Vaccinium bracteatum in MS basic medium.The highest induction rate of adventitious buds was97.7%,and the medium was WPM+ZT 2.0 mg·L^-1.The medium in which the potato diced was added significantly promoted the elongation of the cluster adventitious buds,so the medium suitable for the elongation of the adventitious buds was WPM+ZT 1.0 mg·L^-1+potato diced 100 g·L^-1.The rooting effect of the elongated buds was better with 1/2 WPM + NAA 1.0 mg·L^-1+ activated carbon 500 mg·L^-1 medium,a rooting rate of 94.7%could be achieved after 6 weeks.