Abstract: R-4 strains of Stropharia rugosoannulata were used as test materials, microbial plate culture method was used, the effects of 12 kinds of nitrogen sources and 8 kinds of carbon sources on the growth of R-4 strains were studied, in order to provide reference for the cultivation of R-4 strains.The results showed that there was no significant difference in the selection of nitrogen and carbon sources for the mycelial growth of Stropharia rugosoannulata（P＞0.5）,12 nitrogen sources and 8 carbon sources selected in the experiment could be used.When yeast extract was used as nitrogen source and glucose was used as carbon source,the colony growth of Stropharia rugosoannulata was better and the mycelial growth rate was faster.When glucose was used as the optimal carbon source,the colony growth rate,colony diameter,growth rate and hyphal growth index were 9.75 mm ·d-1,50.22 mm,1.41 mm·d^-1 and 46.73,respectively.When yeast extract was used as the optimal nitrogen source,The value of indicator described above were 14.12 mm ·d^-1,52.54 mm,2.10 mm·d^-1 and 43.56,respectively.The results showed that glucose and the yeast extract was the best carbon source and nitrogen source respectively for the growth of Stropharia rugosoannulata.