Abstract: Taking the Fritillaria hupehensis Hsiao disease samples as the specimen, and tissue separation method and mycelium growth rate method were used, the pathogen species of F.hupehensis Hsiao was identified and the effects of different fungicides on the pathogen was studied, in order to provide reference for the effective prevention and control of gray mold on F.hupehensis Hsiao.The results showed that the pathogen of gray mold on F.hupehensis Hsiao belongs to Botrytis sp.,which was a new species.The optimum environment for mycelial growth of strain BMXTX2 was pH 4 and 20 ℃.Glucose and beef extract were the best carbon and nitrogen sources respectively.The results of toxicity measurement showed that 430 g·L^-1tebuconazole SC,8% flusilazole ME and 500 g·L^-1 fluazinam SC had strong inhibitory effect on the pathogen in the chemical fungicide, with EC₅₀ values of 0.15 mg·L^-1,0.48 mg·L^-1 and 0.98 mg·L^-1,respectively.Among the biological fungicide, 1% osthole EW and 80% ethylicin EC had a good antibacterial effect, with EC₅₀＜10 mg·L^-1,which could be used as candidate agents.To our knowledge, this was the first report of Botrytis sp.causing gray mold on F.hupehensis Hsiao, and the five kinds of fungicides screened in this study will provide reference for the effective prevention and control of gray mold on F.hupehensis Hsiao.