Abstract: Taking Hippophae rhamnoides from 11 regions in Qinghai Province and Tibetan sea buckthorn and ribbed sea buckthorn from Qilian county as test materials,agenetic distance matrix and phylogenetic tree were constructed using chloroplast gene marker rbcLto study the genetic variation of Hippophae rhamnoides in different regions of Qinghai Province,in order to provide reference for the classification of sea buckthorn plants.The results showed that the length of the rbcLregion of all three species of sea buckthorn plants was 1 501bp,and there was no length variation.There were different variation sites in the rbcLgene sequences of three species of sea buckthorn plants,and there were also a small number of variation sites between the rbcL genes of Hippophae rhamnoides in different regions.Cluster analysis with some rbcLgene sequences on NCBI revealed basic consistency between morphological and molecular methods for systematic classification of sea buckthorn.This study provided new ideas and methods for future research on the phylogenetic evolution of sea buckthorn using rbcL sequences,laying the foundation for the development and utilization of sea buckthorn.