Abstract: Pellinus linteus was used as the test material.Seven main materials, namely coarse sawdust, sawdust, wheat straw, peanut straw, corn straw, rape straw and soybean straw were used for Pellinus linteus cultivation.The simple lattice method for mixed material design was used.The formula that main materials inclued coarse sawdust, rape straw and soybean straw was screened.The mycelial growth rate and enzyme activities of laccase, polyphenol oxidase, cellulase, hemicellulase and acid protease were measured.The correlation between mycelial growth rate and extracellular enzymes activities were analyzed, in order to provide a reference for screening the formula of Pellinus linteus cultivation.The results showed that different substrates had different effects for the extracellular enzymes activities.And the contingency between extracellular enzymes activities and mycelial growth rate was poorer.The optimized formula included 41.5% coarse sawdust, 41.9% rape straw, 16.6% soybean straw, 20% wheat bran, 1% sucrose, 1% calcium carbonate.