Abstract: Taking Isatis indigotica Fort.seeds as the test material, the effect of different mass fractions of PEG 6000 at different temperatures on the germination and seedling growth of Isatis indigotica Fort.seeds was studied using a culture dish germination method, in order to provide reference for promoting the growth of Isatis indigoatis under unsuitable conditions.The results showed that initiator treatment had effects on seed germination rate, germination potential, germination index, seedling height and relative electrical conductivity.At the same temperature, the germination rate of Isatis indigotica Fort.seeds was decreased with the increase of PEG 6000 concentration, but the germination rate was higher than that of control group（CK） without initiator.With the increase of temperature, the germination period of seeds was shortened.After using initiator at low temperature（10 ℃）,the germination potential and relative electrical conductivity of seeds were significantly different from those of the control group.It could be inferred that the application of PEG 6000initiator could prevent the freezing injury of Isatis indigotica Fort.seedlings,and the optimal mass fraction was10%,which was conducive to the emergence of uniform,healthy growth,and improve the stress resistance ability of seedlings.