Abstract: Taking wolfberry cultivars ‘Ningqi No.1’ and ‘Ningqi No.7’,wolfberry strains ‘1401’ and‘1402’ as the test materials.The methods of growth index investigation and photosynthetic characteristics determination were used to analysis the differences of plant growth and photosynthetic characteristics of four Lycium barbarum L.cultivars（strains）under the root-limited cultivation.In order to explore the morphological growth and photosynthetic characteristics of different Lycium barbarum L. cultivars（strains） in root-limited cultivation.The results showed that ‘1401’ and ‘1402’ had better growth indexes relative to the other two cultivars ‘Ningqi No.1’‘Ningqi No.7’.The net photosynthetic rate（Pn）,intercellular CO₂ concentration（Ci）,transpiration rate（Tr）,stomatal conductance（Gs） were higher than ‘Ningqi No.1’ and ‘Ningqi No.7’.The SPAD and water use efficiency（WUE） of ‘1401’ were the lowest.Through comprehensive analysis, it is concluded that ‘1402’ is more suitable for the root-limited cultivation.