Abstract: Taking Adiantum capillus-veneris as test material,the optimization method of combining single factor experiments and response interviews was used to optimize the deproteinization process of polysaccharides from Adiantum capillus-veneris.The effects of polysaccharides from Adiantum capillus-veneris before and after deproteinization were measuredα-glucasidase inhibitory activity of glucosidase and its ability to scavenge DPPH free radicals were expected,in order to provide reference for the comprehensive utilization of polysaccharides from Adiantum capillus-veneris.The results showed that the optimal deproteinization process conditions of Adiantum capillus-veneris polysaccharides were as follows,the volume ratio between polysaccharide solution and Sevage reagent was 2∶1,the deproteinization time was 10minutes,the deproteinization time was 5times,the protein removal rate could reach 73.41%,and the polysaccharide loss was 25.92%.The IC₅₀of undeproteinized Adiantum capillus-veneris polysaccharides forα-glucosidase and DPPH radicals were0.31,0.81mg·mL^-1,respectively.The IC₅₀of clematis Adiantum capillus-veneris polysaccharides forα-glucosidase and DPPH radicals were 0.26,0.59 mg·mL^-1,respectively,which increased by0.05,0.22mg·mL^-1 compared with undeproteinized proteins,respectively,which proved that the removal of protein by Sevage method could effectively improve the ability of Adiantum capillusveneris polysaccharides to inhibitα-glucosidase and antioxidant capacity.