Abstract: Lycopersicon esculentum Mill and Yunnan purple skin garlic were used as experimental materials, and two methods of rotation garlic（SF） and rotation garlic with simultaneous application of microbial organic fertilizer（SYF） were adopted, the effects of garlic rotation on Lycopersicon esculentum Mill plant growth, fruit quality, rhizosphere soil microorganism and soil enzyme activity were studied, in order to provide reference for alleviating the obstacles of continuous cultivation of protected tomato.The results showed that compared with continuous cropping FF treatment.SYF treatment could improve the root activity of Lycopersicon esculentum Mill, while SF treatment could inhibit the root activity, the susceptible rate of SYF and SF treatment decreased by 61.54% and SYF treatment by 84.62%.Compared with FF,SF and SYF treatment increased 50.00% and 41.51% in April, 18.24% and 97.85% respectively in May.The contents of vitamin C and soluble sugar in Lycopersicon esculentum Mill were not different, but the contents of organic acids in SF and SYF treatment were higher than that in FF treatment.The number of bacteria and actinomycetes in the rhizosphere soil of garlic rotation was lower than that of FF treatment in the middle and late period of rotation, and the number of fungi in the latter period increased obviously, the number of fungi treated by SF and SYF was 24 and 32 times that treated by FF.The activities of amylase and urease increased gradually with the time of Lycopersicon esculentum Mill cultivation, while the activities of sucrase, protease, phosphatase and catalase increased first and then decreased, SYF treatment had the highest soil enzyme activity in this experiment.In conclusion, garlic rotation promoted the growth and yield of Lycopersicon esculentum Mill plants, but had no significant effect on fruit quality, and the rotation of Lycopersicon esculentum Mill and garlic had significant effect on alleviating the obstacles of continuous cropping of protected Lycopersicon esculentum Mill.