Abstract: Taking ‘Yanfen 209’ tomato as test material,high-throughput sequencing technology was used to analyze the effects of liquid organic fertilizer partial replacement of chemical flushing fertilization on the diversity,structural composition and differences of fungal community in tomato root zone soil samples,in order to provide a reference basis for reasonable fertilization of protected tomato production.The results showed that,at 97% similarity level,301 OTU,8 phylum,20 classes,41 orders,78 families,118 genus and 159 species were found.There were 27 total OTU values in the three treatments and the control samples,and there were 106,34 and 16 OTU values in the three treatments,respectively.It was the highest diversity and richness of fungi in T R30 treatment soil sample.The dominant phylum were Basidiomycota,Ascmycota and Mortierellomycota.The dominant genus were unclassifiled_fCeratobasidiaceae,Mortierella and Mycothermus.Through cluster analysis,principal component analysis and non metric multi-dimensional scale analysis,it was found that there were high similarity of fungal colony composition and greatest commonness between the control sample and T R30 treatment sample.The comprehensive analysis showed that liquid organic fertilizer instead of chemical flushing fertilization could increase the species of exogenous fungi in root zone soil,and change the richness,diversity and colony composition,and T R30 treatment has obvious advantages.