高级检索+

一种黑木耳菌包中病原菌的分离鉴定及其生物学特性

Isolation, Identification and Biological Characteristics of Pathogen in Auricularia heimuer Bag

  • 摘要: 以哈尔滨市工厂化生产的黑木耳菌包中分离纯化的1株病原菌“ZJ-01”为试材,采用形态学和分子生物学方法鉴定“ZJ-01”,研究了碳源、氮源、pH和温度对“ZJ-01”菌丝生长速度和长势的影响,以期为病原菌“ZJ-01”的防治提供参考依据。结果表明:“ZJ-01”菌落和菌丝体形态特征与白囊耙齿菌(Irpex lacteus)相似。ITS序列与白囊耙齿菌同源性最高,为99.30%~99.47%,系统发育树分析发现“ZJ-01”与I.lacteus聚在同一个分支上,其中与I.lacteus strain xsd08033(EU918701.1)亲缘关系最近。结合形态学和ITS序列结果将“ZJ-01”归属为白囊耙齿菌。柯赫氏法则验证表明“ZJ-01”为引起黑木耳菌包污染的病原菌。在试验范围内白囊耙齿菌“ZJ-01”菌丝体生长的最佳碳源为麦芽糖,氮源为酵母浸粉,pH为5~6,温度为30℃。

     

    Abstract: The pathogenic ‘ZJ-01’, isolated and purified from Auricularia heimuer bag production factory in Harbin, was selected as the experimental material.The morphological and molecular biology methods were used to identify taxonomic status of ‘ZJ-01’.The effects of carbon source, nitrogen source, pH, and temperature on the mycelium growth rate and growth status of ‘ZJ-01’ were studied, in order to provide reference for the prevention and control of ‘ZJ-01’.The results showed that the colony and mycelium morphology of ‘ZJ-01’ was similar to those of Irpex lacteus.The ITS sequence homology of ‘ZJ-01’ with I.lacteuswas from 99.30% to 99.47%.‘ZJ-01’ had the closest genetic relationship with I.lacteus strain xsd08033(EU918701.1).‘ZJ-01’ was identified belong to I.lacteus based on morphology and ITS sequence analysis.Koch′s postulates proved that ‘ZJ-01’ was the pathogen that caused contamination of A.heimuer bag.Researches on growth characteristics showed that the best carbon source for ‘ZJ-01’ mycelial growth was maltose,and the nitrogen source was yeast extrat powder,pH of 5to 6,and temperature of 30 ℃.

     

/

返回文章
返回