Abstract:
The pathogenic ‘ZJ-01’, isolated and purified from Auricularia heimuer bag production factory in Harbin, was selected as the experimental material.The morphological and molecular biology methods were used to identify taxonomic status of ‘ZJ-01’.The effects of carbon source, nitrogen source, pH, and temperature on the mycelium growth rate and growth status of ‘ZJ-01’ were studied, in order to provide reference for the prevention and control of ‘ZJ-01’.The results showed that the colony and mycelium morphology of ‘ZJ-01’ was similar to those of Irpex lacteus.The ITS sequence homology of ‘ZJ-01’ with I.lacteuswas from 99.30% to 99.47%.‘ZJ-01’ had the closest genetic relationship with I.lacteus strain xsd08033(EU918701.1).‘ZJ-01’ was identified belong to I.lacteus based on morphology and ITS sequence analysis.Koch′s postulates proved that ‘ZJ-01’ was the pathogen that caused contamination of A.heimuer bag.Researches on growth characteristics showed that the best carbon source for ‘ZJ-01’ mycelial growth was maltose,and the nitrogen source was yeast extrat powder,pH of 5to 6,and temperature of 30 ℃.