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盐胁迫下PGPR菌剂对黑麦草植株生理特性的影响

Effects of PGPR Bacteria on Physiological Characteristics of Ryegrass Plants Under Salt Stress

    摘要
  • 摘要: 以黑麦草为试材，采用盆栽试验法，研究了不同浓度NaCl处理下接种植物根际促生菌芽孢杆菌CP5（T1）、LM2（T2）、T3（混合菌剂，T1∶T2=1∶1）对黑麦草生理特性的影响，以期为PGPR菌剂的应用提供参考依据。结果表明：1)接种T1菌剂能在较高浓度(500、700 mmol·L-1)盐胁迫下降低丙二醛（MDA）含量、提高可溶性糖含量及过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性；2)接种T2菌剂后在较高盐浓度(500、700 mmol·L-1)胁迫下降低黑麦草MDA含量，提高可溶性糖含量及过氧化物酶（POD）、SOD、CAT活性；3)不同浓度盐分胁迫下，接种T3菌剂均可提高可溶性糖含量、脯氨酸（Pro）含量、POD、SOD、CAT活性，降低MDA含量。尤其是在300 mmol·L-1盐胁迫下，接种T3植株较未接种菌剂组SOD活性显著上升44.42%,500 mmol·L-1盐胁迫下，接种T3植株较未接种菌剂组Pro含量、可溶性糖含量、CAT活性分别显著上升17.42%、100.74%、91.60%,700 mmol·L-1盐胁迫下，接种T3菌剂植株较未接种菌剂组MDA含量显著下降43.83%、POD活性显著上升69.29%（P<0.05）。综上所述，接种根际促生菌芽孢杆菌有助于缓解植物生长时受到的盐胁迫，且混合菌剂缓解盐胁迫的效果显著大于单个菌剂，具有开发应用为盐胁迫环境植物生长菌剂潜能。

     

    Abstract: Taking ryegrass as the test material, a pot experiment was conducted to study the effects of inoculating rhizosphere growth promoting bacteria Bacillus subtilis CP5（T1）,LM2（T2）,T3（mixed bacterial agent, T1∶T2=1∶1） on the physiological characteristics of ryegrass under different concentrations of NaCl treatments, in order to provide reference for PGPR application.The results showed that, 1) inoculation with T1 bacterial agent could reduce MDA content, increase soluble sugar content, CAT activity, and SOD activity under relatively high concentration(500,700 mmol·L-1 NaCl) salt stress.2) After inoculation with T2 bacterial agent, the MDA content of ryegrass was reduced and the soluble sugar content, POD,SOD,and CAT activities were increased under relatively high concentration(500,700 mmol·L-1 NaCl) salt stress.3) Under different concentrations of salt stress, inoculation with T3 bacterial agent could increase soluble sugar content, proline（Pro） content, peroxidase（POD）,superoxide dismutase（SOD）,catalase（CAT） activity, and reduce malondialdehyde（MDA） content.Especially under 300 mmol·L-1 salt stress, the SOD activity of T3 inoculated plants significantly increased by 44.42% compared to the non inoculated group.Under 500 mmol·L-1 salt stress, the Pro content, soluble sugar content, and CAT activity of T3 inoculated plants significantly increased by 17.42%,100.74%,and 91.60% compared to the non inoculated group, respectively.Under 700 mmol·L-1 salt stress, the MDA content of T3 inoculated plants significantly decreased by 43.83% and POD activity significantly increased by 69.29% compared to the non inoculated group（P＜0.05）.In conclusion, this study indicated that inoculation with rhizosphere growth promoting bacteria Bacillus subtilis could help alleviate salt stress on plant growth, and the effect of mixed microbial agents on alleviating salt stress was significantly greater than that of single microbial agents, indicating the potential for development and application as a growth microbial agent for plants under salt stress.

     

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