Abstract: Taking the cultivating soil of Poria cocos by different planting modes as experimental materials, using Illumina novaseq high-throughput sequencing technology to study the changes of soil bacterial community before planting and after sclerotium harvest of Poria cocos forest-medicine bionic cultivation and farmland monoculture cultivation.The function of metagenomewas predicted using Tax4fun, in order to provide reference for the suitable cultivation mode and the avoiding continuous cropping mechanism of Poria cocos.The results showed that the number of soil bacterial OTUs increased slightly after two cultivation modes of Poria cocos.Alpha diversity analysis showed that there was no significant difference among Shannon index, Simpson index, Chao1 index and ACE index.The phyla with the highest relative abundance were Proteobacteria, Acidobacteria, Firmicutes, Verrucomicrobia, Crenarchaeota, Actinobacteria, Bacteroidetes, Chloroflexi, Nitrospirae.There was no significant change in the relative abundance of the above-mentioned departments in the farmland monoculture cultivation.In forest-medicine bionic cultivation, the relative abundance of Acidobacteria Verrucomicrobia and Crenarchaeota increased significantly, but did not reach a very significant level.UPGMA cluster analysis showed that the two cultivation patterns were clustered into one category respectively.Tax4fun function prediction was annotated to 6 functions at the first level, the secondary function prediction included 35 sub functions.In summary, the bacterial community structure of Poria cocos cultivated in different models was relatively stable, the number of bacteria increased only a small amount, and the dominant bacterial community did not change significantly.Soil bacteria might not be the main cause of Poria cocos intolerance to continuous cropping.