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盐胁迫和干旱胁迫对神香草种子萌发的影响

Effects of Salt and Drought Stress on Seeds Germination of Hyssopus officinalis

  • 摘要: 以神香草种子为试材,采用滤纸培养皿法,研究其在不同浓度盐(NaCl、NaHCO3、Na2CO3)溶液、聚乙二醇(PEG 6000)溶液中的萌发特性,测定发芽率、发芽势、发芽指数等指标,并通过相对发芽率与各处理浓度建立的函数方程确定其种子的耐受性,以期为盐碱地区神香草的应用提供参考依据。结果表明:1)随着盐胁迫的增强,神香草种子的发芽率、发芽势和发芽指数均呈下降趋势,萌发起始时间逐渐延迟,发芽速率减慢。2)神香草种子对盐胁迫具有一定适应性,但不同盐溶液对神香草种子萌发的抑制效果具有差异性,神香草种子对中性盐胁迫的耐受性较强,对碱性盐胁迫较敏感。3)神香草种子在轻度干旱条件(PEG 6000 5 g·L-1)下发芽率、发芽势和发芽指数升高,随着PEG 6000溶液浓度的增大,神香草种子发芽起始时间推迟,各项指标呈下降趋势,种子的萌发表现出低促高抑的现象。

     

    Abstract: Taking Hyssopus officinalis seeds as the test material, the germination characteristics of Hyssopus officinalis seeds under different concentrations of salt(NaCl, NaHCO3,Na2CO3) and polyethylene glycol(PEG 6000) solutions were studied by using filter paper culture dish method.Several indexes of seed germination were measured such as germination rate, germination potential and germination index, the tolerance of its seeds was determined through the functional equation established between relative germination rate, in order to provide reference for the application of Hyssopus officinalis in saline alkali areas.The results showed that, 1) with the increasing of salt stress, the germination rate, germination potential and germination index of Hyssopus officinalis seeds were all reduced.The germination initiation time gradually delayed, and the germination rate slowed down.2) Hyssopus officinalis seeds had certain adaptability to salt stress, but the inhibiting effects of different single-salt-alkali solutions on seed germination of Hyssopus officinalis were different.Hyssopus officinalis seeds were more tolerant to salt stress and more sensitive to alkali stress.3)Under mild drought stress(PEG 6000 5 g·L-1),the germination rate, germination potential and germination index of Hyssopus officinalis seeds were gradully increased.With the increasing of PEG 6000 solution concentration, the germination initiation time of Hyssopus officinalis seeds was delayed, and the indexes showed a downward trend.The germination of Hyssopus officinalis seeds showed low promotion and high inhibition.

     

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