Abstract: Based on experimental validation and bioinformatics methods, SSR analysis result were exploited from the genomic of Lepista sordida and 4 strains.In the meantime, 50 SSR were selected for primer design and experimental verification,in order to provide theoretical basis for the germplasm resources identification, variety selection and molecular marker-assisted breeding of L.sordida.The results indicated that L.sordida had 7 SSR types.A total of 2 486 SSR were retrieved, P1 type was the main type, accounting for 67.70% and also higher distribution density than other type.There were more types of four, five and six base repeats.However, the number was small.Four primers with high pair property, repeatability and specificity were obtained from the validation experiment, which were HLMSSR001,HLMSSR004,HLMSSR015 and HLMSSR041.HLMSSR001 and HLMSSR004 could be amplified by a single primer to distinguish and identify the 4 strains of L.sordida.At the same time, the DNA fingerprint coding of 4 pairs of primers was constructed and the unique 8-digit molecular fingerprint database codes of 4 L.sordida strains were obtained.