Abstract: Taking peppers ‘Sujiao 1614’‘Juwuba’‘Xuanmei’ and ‘Latianxia’ as experimental materials, the growth and quality indexes of different pepper varieties were analyzed and determined by soilless cultivation method, in order to provide reference for the selection of excellent pepper varieties.The results showed that at different growth stages, the plant height of ‘Sujiao 1614’ was the highest, followed by ‘Latianxia’.At the fruiting stage, the plant height of ‘Sujiao 1614’ and ‘Latianxia’ were 102.67 cm and 94.67 cm respectively; the stem diameter of ‘Sujiao 1614’ was significantly higher than those of the other three varieties at all growth stages; the SPAD value of ‘Sujiao 1614’ reached 57.03 in the fruiting stage, which was significantly higher than that of other varieties; the content of vitamin C in ‘Latianxia’ was the highest, reached 27.22 mg·（100 g）^-1,which was significantly higher than that of the other three varieties, while the content of nitrate in ‘Latianxia’ was significantly lower than those of other varieties, as low as 98.77 mg·kg^-1;the four varieties were divided into three categories by cluster analysis.The first category was ‘Latianxia’,the second category was ‘Sujiao 1614’,and the third category was ‘Juwuba’ and ‘Xuanmei’;principal component analysis showed that the comprehensive score order of the four kinds of pepper was ‘Sujiao 1614’＞‘Latianxia’＞‘Juwuba’＞‘Xuanmei’.In conclusion, ‘Sujiao 1614’ could be used as an excellent variety for soilless cultivation in Nanjing.