Abstract: The leaves of wild Nervilia fordii（Hance）Schltr.infected with leaf spot disease were used as test materials.The pathogen of the leaf spot of N.fordii was obtained by tissue separation method,and identified by molecular and morphological methods.The pathogen was verified according to Koch′s postulates.Subsequently,the antagonistic effect of endophytic fungi isolated from N.fordii on the growth of the pathogen was detected by plate confrontation method and cross method,in order to explore the pathogen causing leaf spot of Nervilia fordii（Hance）Schltr.,and provide atheoretical basis for effective prevention and treatment of the disease.The results showed that C.canescens was identified as the pathogen of leaf spot in N.fordii reservoir.Then,8 kinds of endophytic fungi with obvious antagonistic effects were screened,with the inhibition rate of 44%.Among the eight strains,1151（Penicillinumsp.）had the best antagonistic effect on the pathogen,up to 59.8%.