Abstract: Taking tomato as the research object,setting four treatments including non-inoculated（CK）,inoculation with BEG141,BEG167 and BEG168.A three-compartment root box culture system was used to separate the total respiratory chamber,intermycelial respiratory chamber and basic soil respiratory chamber with 30 μm and 0.45 μm nylon nets to study the effects of arbuscular mycorrhizal fungi（AMF） on tomato growth and soil each component′s respiratory rate.In order to provide a reference for the influence of arbuscular mycorrhizal on tomato growth and soil respiration rate.The results showed that all the three strains could form a good symbiotic relationship with tomato root system,and the average colonization was 88.90%.Compared with CK,the fresh weight of the underground part inoculated with BEG167 was 6.24 times that of CK treatment,and the chlorophyll content was 2.63 times that of CK treatment.The average respiration rates of the total respiratory chamber,intermycelial respiratory chamber and basal soil respiratory chamber were 26.917,24.662,21.356 g·m^-2·d^-1,which were significantly higher than the respiration rate of CK treatment.During the growth period of tomato,soil respiration rate showed different trends in day and night,but the respiration rate in each chamber under CK treatment was lower than that under inoculation treatment.