Abstract: The genetic diversity,genetic relationship and germination promoting effect of 9 Gastrodia elata germinating fungi and their pollen seeds in Hanzhong area were studied by means of antagonistic experiment,ITS sequence determination,esterase isozyme technology and seed mixing,in roder to provide reference for the management and evaluation of germplasm resources of Gastrodia elata germinating fungi.The results showed that 9 germination fungi were classified into 3 types,insignificant,significant and extremely significant according to the degree of antagonism of each pair;according to the difference of ITS sequence,they were classified into Mycena sp.and Heydenia sp.2;according to esterase isozyme.The zymogram bands could be divided into 5 categories at a similar level of 80%;9 germination strains co-germinate with Gastrodia elata seeds,of which the highest growth rate of C3 strain was （0.30±0.01）cm·d^-1,and the highest germination rate of C2 strain was 64.66%±2.89%,the comprehensive performance of C2 and C3 strains were the best.