Abstract: The ‘Trensor’ of Asiatic lily was used as the test material.The effects of different concentrations of hormones on the scale induction, adventitious bud proliferation and bulb rooting culture of Asiatic lily were studied by the method of plant tissue culture, and the effects of different transplanting substrates on the growth of lilium tissue culture seedlings were studied, in order to provide reference for the establishment of ‘Trensor’ tissue culture rapid propagation system of Lilium asiatica.The results showed that the optimal medium for lilium scale induction was MS+0.5 mg·L^-1 NAA+0.5 mg·L^-1 6-BA+30 g·L^-1 sucrose+6 g·L^-1 agar, and the highest induction rate was 74.44%,which was higher than other treatments.The best proliferation medium was MS+0.1 mg·L^-1 NAA+1.0 mg·L^-1 6-BA+30 g·L^-1 sucrose+6 g·L^-1 agar, and the number of advective buds was the highest with a proliferation coefficient of 3.27.The suitable rooting medium was 1/2MS+0.1 mg·L^-1 NAA+0.3 mg·L^-1 IBA+40 g·L^-1 sucrose+6 g·L^-1 agar, and the average rooting number was 14.33.It was 1.15-2.02 times more than other treatments.After transplanting, the survival rate of tissue culture seedlings was 87.50% in the matrix ratio of peat and perlite was 1∶1,and the growth of tissue culture seedlings was the best.