Abstract: Taking three chrysanthemum varieties（‘Huihuang’ ‘Boerduohong’ and ‘Huangfurong’） as the test materials, a seedling cultivation experiment was conducted by adding cow manure compost in different proportions（with a volume ratio of 1∶1,3∶1,and 5∶1,respectively）,the effects of adding different volumes of cow manure compost on the physicochemical properties of the cultivation substrate, as well as the morphology, physiological indicators, and bacteriostatic effect of potted chrysanthemums were studied, in order to reduce the incidence of potted chrysanthemum disease and improve its ornamental value.The results showed that the matrix bulk weight, the content of alkali-hydrolyzed nitrogen, available phosphorus and available potassium, the pH increased with the increase of compost proportion of cow manure, while the porosity decreased, the EC value did not change significantly.The addition of cow manure compost in the substrate could significantly promote the elongation of plant height, the thickening of flower stem, the enlargement of crown width and the growth of leaves of potted chrysanthemum.The three kinds of potted chrysanthemum had the highest disease index and the lowest soluble protein content in the control group.The survival rate of potted chrysanthemum in treatment group was 100%.‘Huihuang’ grew best under Y1 treatment, ‘Boerduohong’ and ‘Huangfurong’ grew best under Y3 treatment.‘Huihuang’ had the lowest disease index in Y1 and Y2 treatment, and ‘Boerduohong’ and ‘Huangfurong’ had the lowest disease index in Y3 treatment.Therefore, adding cow manure compost to the substrate could promote the growth of potted chrysanthemum and reduce its risk of disease.