Abstract: Fusarium oxysporumf.sp.cucumerinum was used as the target fungal,and the fungal strain isolated and purified from the soil was used as the test strain.The plate confrontation method was used for primary screening and the inhibition rate of the fermentation broth was used for re-screening.Combined with morphological and molecular biological method,the inhibitory effect of fungi isolated from soil on cucumber Fusarium wilt was studied,and the antagonistic strains were screened and identified,in order to provide a theoretical basis for the biological control of cucumber Fusarium wilt.The results showed that there were six strains with obvious antagonistic effect,among which strain 14 had the highest inhibition rate of 70.83%.The phylogenetic tree was constructed and strain 14 was confirmed as Talaromyces funiculosus.