Abstract: Taking ‘Qianjiao No.11’ peper as test material, the effects of humic acid liquid on yield, quality, nutrient accumulation and fertilizer use efficiency of fresh pepper ‘Qianjiao No.11’ in Guizhou were studied by field experiment.The effect of humic acid liquid fertilizer on the biological effect and fertilizer utilization rate of fresh pepper in Guizhou Province was explored, in order to provide a reference for the application of humic acid liquid fertilizer in pepper production in Guizhou Province.The results showed that compared with FP treatment, CSF,CLF1 and CLF2 treatments significantly increased the fresh yield of pepper by 6.12%,9.12% and 2.30%,respectively.But LF treatment decreased by 6.99%.The protein and vitamin C contents of CSF,CLF1 and CLF2 treatments were increased to some extent, but they were decreased in the LF treatment.From the fertilizer use efficiency, the agronomic efficiency and apparent use efficiency of nitrogen and phosphorus could be improved in the CSF,CLF1,CLF2 and LF treatments, which the CLF1 treatment was the best.The apparent use efficiency of nitrogen, phosphorus and potassium were 39.01%,14.68% and 46.54% respectively in the CLF1 treatment.In the cultivation of fresh pepper in Guizhou, the application of compound fertilizer as base fertilizer and humic acid liquid fertilizer as topdressing can not only increase the yield and quality of pepper, but also reduce and increase the efficiency of fertilizer.