Abstract: Taking the dried fruits of ‘Ningnongqi No.15’,a new strain of Lycium barbarum L.as experimental materials, the effects of different tree shapes and picking periods on the nutritional quality contents in dried fruits were studied by means of variance analysis and principal component analysis, in order to find out the most suitable tree shape for the accumulation of nutrients in ‘Ningnongqi No.15’ fruit.The results showed that there were significant differences in the contents of total sugar, flavonoids and carotenoids in fruits of different tree shapes and picking periods, and the effects of the two on the contents were interactive.Lycium barbarum polysaccharide content in dried fruits was significantly different only in different picking periods, which was the main effect factor.The betaine content was only significantly different under different picking periods of the trunk shape and under different tree shapes on June 29,but there was an interaction effect between the two factors on the content.As time went by, the content of total sugar in dried wolfberry fruits increased gradually, the content of flavonoids decreased gradually, the content of Lycium barbarum polysaccharide showed a trend of first decreasing and then increasing, while betaine and carotenoid both showed a trend of increasing first and then decreasing.Under different tree shape conditions, the contents of total sugar, Lycium barbarum polysaccharides and carotenoids were all in the form of trunk layered tree shape ＞ trunk tree shape ＞ single trunk double arm tree shape, betaine content in the trunk tree shape ＞ single trunk double arm tree shape ＞ trunk layered tree shape, flavonoids content in the trunk tree shape ＞ trunk layered tree shape ＞ single trunk double arm tree shape.Finally, the principal component analysis method was used for comprehensive evaluation, and the scores from high to low were trunk tree shape（0.702） ＞ trunk layered tree shape（0.649） ＞ single trunk double arm tree shape（-1.351）,that was, the trunk tree shape was most favorable for nutrient quality formation of ‘Ningnongqi No.15’ fruit.