Abstract:
Four Ganoderma lingzhi strains were used as test materials, PDA-guaiacol medium, PDA-aniline blue medium, cellulase-producing screening medium and xylanase-producing screening medium were used to determine the production capacity of LCC in these strains.The mycelial growth rate, the activity of lignocellulose degrading enzyme and the degradation of lignocellulose in corn stalk were studied in different cultures during the bacterial development stage, in order to provide reference for the study of lignocellulose utilization in Ganoderma lingzhi during the bacterial development stage.The results showed that LZ-10 with high Lignocellulase activity was screened by 4 kinds of medium and used in the follow-up experiment.In the degradation experiment of corn straw, with the increase of the proportion of corn straw in formula 1 to 7,the mycelial growth rate showed a trend of first increasing and then decreasing, in which formula 1 reached the maximum value of(5.72±0.12)mm·d
-1.There were differences in lignocellulosic enzyme activity and lignocellulosic degradation rate among different formulations during the growth period of Ganoderma lingzhi.In formulations 5 and 6,the degradation rate of lignin reached 43.08%±0.05%,and the activities of laccase and manganese peroxidase were the highest.In formulations 5 and 6 with the highest xylanase activity, the maximum degradation rate of hemicellulose reached 36.20%±0.23%,while the degradation rate of cellulose did not change significantly.In summary, the mycelial growth was slow when the added amount of corn stalk was 80%-90% during the period of Ganoderma lingzhi development, but the utilization of lignocellulose was more comprehensive.