Abstract:
Taking the scale of Lilium davidii var. unicolor test materials, the air culture method was selected for single-factor screening test and two-factor randomized block test, to explore the effect of Lilium davidii var. unicolor scales on the reproduction of bulblet under different temperature and light, in order to screen the most suitable Lilium davidii var. unicolor scales for air culture environmental factors.The results showed that the best culture effect(decay rate, rooting rate, differentiation rate) and bulblet culture effect(differentiation number, rooting number, bulblet quality, bulblet grading) under different aeroponic temperature conditions was achieved by 25 ℃ treatment, in which the highest rate of scale differentiation reached 89.81% and the differentiation number of bulblet was 146.48 grains·(100 pieces)
-1,followed by 20 ℃ treatment, in which the differentiation number of bulblet reached 146.67 grains·(100 pieces)
-1.The rooting rate of scales at 25 ℃ and 20 ℃ reached 60.0%.The results of the two-factor randomized group test showed that the highest number of bulblet differentiation was 278 grains·(100 pieces)
-1 and the highest number of roots was 424.33 strips·(100 pieces)
-1 under the A
2B
1 combination, in which the effects of temperature on differentiation rate, rooting rate, bulblet mass and bulblet grading(>4 mm) were significant.The effect of light on the number of bulblet differentiated was significant and the effect on the quality of bulblet was highly significant.Correlation analysis among aeroponics indicators showed that differentiation rate was negatively correlated with decay rate, while rooting rate was positively correlated with differentiation rate and differentiation number, and rooting number was positively correlated with differentiation number and rooting rate.The cumulative variance contribution of the first two components in the principal component analysis reached 86.087 6%,among which differentiation rate, differentiation number, rooting rate and bulblet mass could be used as the main evaluation index.The aeroponic effect of eight temperature and light combinations was evaluated and the highest score was obtained from combination A
2B
1.20-25 ℃ was the most suitable temperature for bulb cultivation and the best aeroponic effect was obtained at 25 ℃ and 500 lx, which can be promoted and applied in the large-scale propagation and production of Lilium davidii var. unicolor using aeroponics.