Abstract: Chrysanthemum morifolium ‘Niukou’（Green） were used as materials.The explants were treated by different sterilization methods, and the leaf adventitious bud induction, proliferation culture and rooting culture were carried out by adding different concentrations and different kinds of plant hormones, so as to screen the best explants, disinfection methods, and the best formula of induction, proliferation and rooting medium, in order to establish an efficient regeneration system of ‘Niukou’（Green）.The results showed that the optimum disinfection method was 0.1% HgCl₂ for 9 minutes after 75% alcohol for 30 seconds, the contamination rate was 6.67% and the mortality rate was 0%.The most suitable culture-medium formulations for callus and adventitious buds induced by leaf discs were, MS+1.00 mg·L^-1 6-BA+0.5 mg·L^-1 NAA,under such circumstances, callus induction rate was 100%,and callus showed light green, loose and granular, while adventitic bud induction rate was 91.11%,and bud clusters were dense, well grown and dark green in color.The most suitable rooting medium was adding 0.3 mg·L^-1 NAA to MS.