Abstract: Taking Dysosma versipellis（Hance） M.Cheng which is an endemic and endangered medicinal plant in China as the test material, the effects of different plant hormones on callus formation, embryoid induction and plant regeneration of Dysosma versipellis（Hance） M.Cheng were studied by orthogonal design of DPS software and Duncan′s multiple range test of SPSS,in order to provide a reference for the rapid induction of callus, embryoid and plant regeneration of D.versipellis under in vitro culture conditions, as well as the in-depth study of the mechanism of action, signal regulation mechanism and artificial seed production of podophyllotoxin accumulation induced by fungal elicitors in D.versipellis.The results showed that leaves and petioles were the most suitable materials in inducing callus.The effect of phytohormone on callus formation followed the order of 2,4-dichlorophenoxyacetic acid（2,4-D）＞thidiazuron（TDZ）＞kinetin＞naphthylacetic acid（NAA）＞2-ip.The best medium for callus formation was MS+2,4-D 1 mg·L^-1+NAA 0.05 mg·L^-1+TDZ 0.5 mg·L^-1+2-ip 1 mg·L^-1.The optimal medium to induce the formation of granular callus embryoid was MS+6-BA 0.5 mg·L^-1+NAA 0.1 mg·L^-1,and the induction rate was 71.33%.The embryoid rooting and plant regeneration medium was MS+IBA 0.5 mg·L^-1+GA₃ 0.5 mg·L^-1.