Abstract: Lychnis cognata Maxim was used as the experimental material, and the L.cognata was inoculated by using three arbuscular mycorrhizal fungi（AMF） species including Funneliformis mosseae（GM）,Glomus versiforme（GV） and Rhizophagus intraradices（GI） under greenhouse conditions.The effects of inoculation by the three AMF species on morphology, growth performance and root infection of L.cognata were studied, in order to provide a reference for using AMF to promote the cultivation and resource development of wild ornamental plants under artificial conditions.The results showed that all the three AMFs could form mycorrhizal symbiotic structure with L.cognata.The root infection rates of GV,GI and GM were 35.71%,23.00% and 17.50%,respectively.However, there was no significant difference in the mycorrhizal dependence among the three species of AMF.There were no significant differences in plant height, aboveground biomass, underground biomass, total biomass and root-shoot ratio of L.cognata inoculated with AMF compared with the control.The branching number of L.cognata significantly increased when inoculated by GI.In addition, three species of AMF had no significant effects on chlorophyll content, root activity and root structure of L.cognate.In conclusion, although AMF can form mycorrhizal entangling structure with L.cognata under the experimental conditions, it had no significant effect on the growth of L.cognata.The results could provide a reference for further exploration of the interaction between non-mycorrhizal plants and AMF.