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土壤盐碱胁迫程度对番茄光合特性的影响

Effects of Soil Saline-alkali Stress Degree on Photosynthetic Characteristics of Tomato

  • 摘要: 以C2和C5 2个不同抗性番茄种质为试材,采用盆栽土培的方式,设置3个不同胁迫程度盐碱土处理,以非盐碱胁迫土壤为对照,研究了不同盐碱胁迫程度对2个番茄品种光合气体交换参数,以及对叶片快速叶绿素荧光动力学曲线及参数的影响,以期为进一步全面解析番茄耐盐碱机制和抗盐碱育种提供参考依据。结果表明:盐碱胁迫通过对番茄叶绿素合成的抑制,对光合气体交换参数产生影响,从而降低净光合速率;盐碱胁迫降低了2个番茄品种最大光化学效率、实际光化学效率、光合电子传递速率、光化学淬灭系数;同时盐碱胁迫改变了2个品种番茄OJIP曲线形状。综上可知,盐碱胁迫会导致C2和C5品种PSⅡ反应中心活性、电子传递效率和光化学效率降低,OEC和PSⅡ受体侧受到损伤,叶绿素含量下降,从而使净光合速率降低。在盐碱胁迫条件下,C2品种较C5品种具有较高的PSⅡ反应中心活性和电子传递速率,提高净光合速率,以及通过耗散过量光能,减少盐碱胁迫带来损害,因此生长受抑制程度显著低于C5品种。

     

    Abstract: Taking C2 and C5 tomato germplasm with different resistance as experimental materials, pot experiments were carried out and three saline-alkali soil treatments with different stress degrees were set up.Compared with non-saline-alkali stressed soil, the effects of different saline-alkali stress degrees on photosynthetic gas exchange parameters of two tomato varieties, as well as the rapid chlorophyll fluorescence kinetic curve and parameters of leaves were studied, in order to provide reference for further comprehensive analysis of tomato salt-alkali tolerance mechanism and salt-alkali resistance breeding.The results showed that saline-alkali stress reduced the net photosynthetic rate and affected the parameters of light and gas exchange by inhibiting the synthesis of chlorophyll in tomato.Saline-alkali stress reduced the maximum photochemical efficiency, actual photochemical efficiency, photosynthetic electron transfer rate and photochemical quenching coefficient of the two tomato varieties.At the same time, saline-alkali stress also changed the shape of OJIP curve of two tomato varieties.To sum up, saline-alkali stress would reduce the activity, electron transfer efficiency and photochemical efficiency of PSⅡ reaction center of C2 and C5 varieties, harm OEC and PSⅡ receptor side, reduce chlorophyll content, and thus reduce the net photosynthetic rate.In case of saline-alkalitress, C2 variety had higher PSⅡ reaction center activity and electron transfer rate than C5 variety, improved the net photosynthetic rate, and reduced the damage caused by saline-alkali stress by dissipating excess light energy, so the growth inhibition degree was significantly lower than C5 variety.

     

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