Abstract: Six Lycium barbarum clones were used as experimental materials and treated with the same IBA concentration to analyze the rooting process of cuttings and the changes in the contents of nutrients and enzyme activities during the rooting process, in order to explore the mechanism of rooting and promote the promotion and application of Lycium barbarum in production practice.The results showed that the rooting types of HT2017-01,HT2017-02,HT2017-05 and HT2017-06 were callus rooting type, and HT2017-03 and HT2017-04 were skin rooting type.Among the six Lycium barbarum clones, HT2017-02 had the highest rooting rate, average root number and rooting index, which was significantly better than other clones, and HT2017-05 had the longest average root length.The changes of nutrient and oxidase activities in cuttings were different.The soluble sugar content（except HT2017-03）,soluble protein content and PPO activity of six Lycium barbarum clones increased with the formation of callus during cuttage.In adventitious root elongation stage, the soluble sugar content of HT2017-01,HT2017-03 and HT2017-04 increased, while the soluble sugar content of HT2017-02,HT2017-05 and HT2017-06 decreased, while the soluble protein content and PPO activity increased.Except for HT2017-04,all POD showed a trend of rising first, then decreasing, and then rising and decreasing, presenting a form of bimodal curve.