Abstract: The chromosome numbers and karyotypes of eight rose germplasm resources with special traits were studied by de-walling and hypotonic methods.In order to supply cytogenetic information to the utilization of these rose resources for the germplasm innovation and cultivar improvement of modern rose.The results showed that,1) the chromosome numbers of different resources were significantly different.Rosa lichiangensis with half-double petals,Rosa lucidissima,the prickless Rosa banksiae Ait.var.banksiae,Rosa sertata×Rosa rugosa and Rosa xanthina f.xanthina were all diploid（2 n=2 x=14）;Rosa ‘Parks’ Yellow Tea-scented China’ was triploid（2 n=3 x=21）;Rosa‘Dian Hong’ was tetraploid（2 n=4 x=28）.Rosa caninawas pentaploid（2 n=5 x=35）.2) The range of nuclear asymmetry coefficient of these eight resources was 59.1%-66.9%,none satellite was observed in the metaphase nuclei of theseresources.3) The karyotypes of the half-double Rosa lichiangensis,Rosa‘Parks’ Yellow Tea-scented China’,Rosa ‘Dian Hong’,Rosa caninawere all 2 B.Those of the prickless Rosa banksiae var.banksiae,Rosa xanthina f.xanthina were both 2 A.The karyotype of Rosa sertata×Rosa rugosawas 1 A and that of Rosa lucidissimawas 3 A.