Abstract: Taking the ten-year-old walnut（Juglans sigillata）orchards in Changning of Yunnan Province as test material, the（arbuscular mycorrhizal fungi, AMF） community and diversity of five types of intercropping were studied.The results showed that in the orchard of four types of intercropping, and the walnut orchard without intercropping treatment, the roots of walnut were significantly colonized by AMF,with the colonization percentage of 64.63% to 91.81%.From the rhizospheric soil, fifteen species belonging to six genera of AMF were identified, the spore density ranged from 86 to 1 422 spores per 50 g air-dry soil, and Glomus claroideum was the common dominant species.The results of statistical analysis showed that the root AMF colonization and spore density in rhizospheric soil were significantly different among the treatments.The AMF colonization and spore density of orchard intercropped with tea was the highest among the treatments, significantly higher than other three intercropping treatments and the control, whereas that of the orchard intercropped with pea was the lowest.This study indicated that different intercropping and corresponding soil management methods significantly root AMF colonization of walnut was higher and the spore density in the rhizospheric soil was higher.Its also affected the community composition and diversity of walnut orchard, in orchard with less soil tending, the intercropping tea in walnut orchard was beneficial for growth and propagation of AMF,higher amount and diversity of AMF could be effectively maintained.The study can provide scientific basis for the full development of AMF natural potential and the application of mycorrhizal technology in walnut plantation.