Abstract: Different formula of S.rugosoannulata was used.The single-factor completely random block design was used.Every level has been designed 20 replications.To study effects of four kinds of formula of S.rugosoannulata on yield and quality.We made good use of local bagasse resource to obtain higher yield.The result showed that the fruiting body of S.rugosoannulata′s pileus length and pileus perimeter order are No.1＜No.2＜No.3＜No.4 under different level during the same date.The stipe length and diameter order was as the same.The fresh weight and dry weight order were No.1＜No.2＜No.3＜No.4.The stipe′s crude fiber and ash contents were higher than pileus.No.1 was the highest.The stipe′s were 4.25%,12.91%.The pileus′were 5.90%,14.27%.The total soluble sugar,crude protein and total amino acids of pileus were higher than stipe.Its order was No.1＜No.2＜No.3＜No.4.The best culture medium formulas of S.rugosoannulata was wheat 93%,bagasse 5%and gypsum 2%.