Abstract: Taking blueberry ‘Pink Blue’ in Wudang District of Guiyang City as the test material, under the condition of 4 ℃,combined with PET+PE composite film packaging, the orthogonal test method was used to study the preservation effect of chitosan, potassium sorbate and ε-polylysine composite coating with different concentrations on blueberry fruit, with the decay rate as the evaluation parameter, in order to provide reference for postharvest storage and preservation of blueberry.The results showed that the formula with better preservation effect was 0.05 g·kg^-1 ε-polylysine, 0.2% potassium sorbate and 2.0% chitosan, which could effectively reduce the decay rate of blueberry during storage, slow down the changes of vitamin C,soluble solids and anthocyanin content, effectively prolong the shelf life of blueberry.